Fibrillin 1 Polyklonaler Antikörper

Fibrillin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29425-1-PBS

Synonyme

FBN1, Asprosin, FBN, Fibrillin-1, MASS

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  WB analysis using 29425-1-AP (same clone as 29425-1-PBS)

  HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 29425-1-AP (same clone as 29425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29425-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Fibrillin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Vollständiger Name: fibrillin 1
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: BC146854
• Gene symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).