Fibrinogen Alpha Chain Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Fibrinogen Alpha Chain Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (2)

Kat-Nr. : 20645-1-AP

Synonyme

FGA, Fib2, Fibrinopeptide A

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• 

  WB analysis of mouse liver using 20645-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20645-1-AP (Fibrinogen alpha chain antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolongewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

20645-1-AP bindet in WB, IHC, IF, IP, ELISA Fibrinogen Alpha Chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibrinogen Alpha Chain fusion protein Ag9567
• Vollständiger Name: fibrinogen alpha chain
• Berechnetes Molekulargewicht: 866 aa, 95 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC020764
• Gene symbol: Fibrinogen Alpha Chain
• Gene ID (NCBI): 2243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.; Authors - Di-Wei Zheng
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Aging (Albany NY); Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.; Authors - Wenjing Wang
mouse	WB	J Cell Mol Med; Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice; Authors - Guillaume Postic
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	IF	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (07-15-2025)

Immunohistofluorescence (PFA-fixed cryosections) image of Fibrinogen Alpha Chain stained mouse stroke brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Mouse Brain

Fibrinogen Alpha Chain Antibody Immunofluorescence validation (1/200 dilution) in Mouse Brain (Cat no:20645-1-AP)

FH

Lillian (Verified Customer) (05-27-2025)

Labeled fibrinogen in our tissue samples. Kept at -20C for the past year and worked even when not aliquoted.

• Applications: Immunohistochemistry
• Cell Tissue Type: Choroid plexus