Fibulin 5 Polyklonaler Antikörper

Fibulin 5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29992-1-PBS

Synonyme

---

• 

  WB analysis using 29992-1-AP (same clone as 29992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29992-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

29992-1-PBS bindet in WB, IHC, Indirect ELISA Fibulin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibulin 5 fusion protein Ag30835
• Vollständiger Name: fibulin 5
• Berechnetes Molekulargewicht: 448 aa, 50 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC022280
• Gene symbol: Fibulin 5
• Gene ID (NCBI): 10516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.