Fibulin 5 Monoklonaler Antikörper

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• KD/KO Validated

Fibulin 5 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5C8

Kat-Nr. : 60081-1-PBS

Synonyme

FBLN5, 2A5C8, Dance, Developmental arteries and neural crest EGF-like protein, EVEC

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  WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

  WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 60081-1-Ig (same clone as 60081-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60081-1-Ig (same clone as 60081-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 60081-1-Ig (same clone as 60081-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60081-1-Ig (same clone as 60081-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60081-1-PBS bindet in WB, IF/ICC, ELISA Fibulin 5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fibulin 5 fusion protein Ag2859
• Vollständiger Name: fibulin 5
• Berechnetes Molekulargewicht: 448 aa, 50 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC022280
• Gene symbol: Fibulin 5
• Gene ID (NCBI): 10516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.