Ficolin 2 Polyklonaler Antikörper

Ficolin 2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32442-1-PBS

Synonyme

FCN2, FCNL, Ficolin B, Ficolin beta, Hucolin

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  WB analysis using 32442-1-AP (same clone as 32442-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32442-1-AP (FCN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

32442-1-PBS bindet in WB, IHC, Indirect ELISA Ficolin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ficolin 2 fusion protein Ag37525
• Vollständiger Name: ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-35 kDa
• GenBank-Zugangsnummer: NM_004108
• Gene symbol: FCN2
• Gene ID (NCBI): 2220
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles in the immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin 2 recognizes pathogen-associated pattern molecules (PAMPs) on the surface of pathogenic microorganisms, activates the complement lectin pathway, and binds to mannose-binding lectin-associated serine proteases (MASPs) to exert immune defense function (PMID: 21315829).