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Ficolin 3 Polyklonaler Antikörper

Ficolin 3 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11867-1-PBS

Synonyme

Hakata antigen, HAKA1, FCNH, FCN3, Collagen/fibrinogen domain-containing protein 3

Filter:
  • Western Blot (WB) analysis of human plasma using Ficolin 3 Polyclonal antibody (11867-1-AP)

    WB analysis of human plasma using 11867-1-AP (same clone as 11867-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 11867-1-AP (Ficolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ficolin 3 Polyclonal antibody (11867-1-AP)

    IF Staining of HepG2 using 11867-1-AP (same clone as 11867-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ficolin 3 antibody (11867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11867-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11867-1-PBS bindet in WB, IF/ICC, Indirect ELISA Ficolin 3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Ficolin 3 fusion protein Ag2449
Vollständiger Name ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 32 kDa
GenBank-ZugangsnummerBC020731
Gene symbol Ficolin-3
Gene ID (NCBI) 8547
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles within the innate immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin-3 (Hakata antigen or H-ficolin) was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus (PMID: 1859827). It has been shown to have a calcium-independent lectin activity. Ficolin-3 is associated with MASPs and sMAP, and which activates the lectin pathway (PMID: 11907111). Ficolin-3 shows affinity for GalNAc, GlcNAc, D-fucose and galactose (PMID: 30868077).