DYKDDDDK Tag Monoklonaler Antikörper

DYKDDDDK Tag Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, CoIP, ELISA

Konjugation

HRP

Publikationen(31)

CloneNo.

8H6A10

Rezensionen (1)

Kat-Nr. : HRP-66008

Synonyme

DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag

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  WB analysis of Recombinant protein using HRP-66008

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66008 (DYKDDDDK Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 24 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

HRP-66008 bindet in WB, CoIP, ELISA DYKDDDDK Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DYKDDDDK Tag
• Konjugation: HRP
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
	WB	Mol Cancer; Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity; Authors - Can Can
		Ann Rheum Dis; Lupus and inflammatory bowel disease share a common set of microbiome features distinct from other autoimmune disorders; Authors - Hao Zhou
	WB	Nat Commun; A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infection; Authors - Wanbo Tai
	WB	Nat Commun; FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.; Authors - Tingxiang Yan
		PLoS Pathog; Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus; Authors - Ming Guo
	WB	Int Immunopharmacol; ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3; Authors - Danhui Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chunxu (Verified Customer) (01-31-2025)

Significant facility the process of WB and this antibody results great specifity

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T, SKOV3, A2780