DYKDDDDK tag Monoklonaler Antikörper

DYKDDDDK tag Monoklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(523)

CloneNo.

8H6A10

Rezensionen (4)

Kat-Nr. : 66008-4-Ig

Synonyme

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis using 66008-4-Ig

  HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

• 

  WB analysis using 66008-4-Ig

  HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading

• 

  WB analysis using 66008-4-Ig

  HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

• 

  IP experiment of Transfected HEK-293 using 66008-4-Ig

  IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug.

• 

  IF Staining of Transfected HEK-293 using 66008-4-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3).

• 

  FC experiment of Transfected HEK-293 using 66008-4-Ig

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected HEK-293 cells
• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 371 publications below
• IHC: See 1 publications below
• IF: See 53 publications below
• IP: See 124 publications below
• CoIP: See 69 publications below
• ChIP: See 9 publications below
• RIP: See 2 publications below

Produktinformation

66008-4-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Affe, Huhn, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DYKDDDDK tag
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
	WB,IP	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
	WB,IP	Nat Metab; Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C; Authors - Yang Li
	ChIP	Blood; Functional, structural and molecular characterizations of leukemogenic driver MEF2D-HNRNPUL1 fusion.; Authors - Ming Zhang
	IP	Nat Commun; Homodimer-mediated phosphorylation of C/EBPα-p42 S16 modulates acute myeloid leukaemia differentiation through liquid-liquid phase separation; Authors - Dongmei Wang
	WB	Nat Commun; UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean; Authors - Jiaqi Sun
	WB	Nat Commun; The stomatin-like protein StlP organizes membrane microdomains to govern polar growth in filamentous actinobacteria under hyperosmotic stress; Authors - Xiaobo Zhong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Arezousadat (Verified Customer) (04-22-2025)

• Applications: Western Blot, Cell culture, Protein purification

FH

Boyan (Verified Customer) (12-12-2022)

worked very well

• Applications: Western Blot, Immunofluorescence

FH

Chun (Verified Customer) (06-19-2022)

This antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000

FH

Céline (Verified Customer) (03-29-2022)

I have compared with anti-Flag M2 from Sigma in an IP experiment and I found the M2 antibody better.

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Hela cells