DYKDDDDK Monoklonaler Antikörper

DYKDDDDK Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

CloneNo.

8H6A10

Kat-Nr. : CL594-66008

Synonyme

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK tag

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  IF Staining of Transfected HEK-293 using CL594-66008

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®594 DYKDDDDK antibody (CL594-66008, Clone: 8H6A10 ) at dilution of 1:200.

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  FC experiment of Transfected HEK-293T using CL594-66008

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope) (CL594-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL594-66008 bindet in IF/ICC, FC (Intra) DYKDDDDK und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DYKDDDDK
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
Human	IF	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu
	IF	Nat Commun; The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis; Authors - Peilu She