Flightless I Polyklonaler Antikörper

Flightless I Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27729-1-AP

Synonyme

FLI, Fli1, Flightless-1, FLII, FLIL, Protein flightless 1 homolog

---

Galerie der Validierungsdaten

• 

  WB analysis using 27729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, T-47D-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27729-1-AP bindet in WB, ELISA Flightless I und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Flightless I fusion protein Ag26865
• Vollständiger Name: flightless I homolog (Drosophila)
• Berechnetes Molekulargewicht: 1269 aa, 145 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC025300
• Gene symbol: FLII
• Gene ID (NCBI): 2314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).