Flightless I Monoklonaler Antikörper

Flightless I Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9C8

Kat-Nr. : 67039-1-PBS

Synonyme

FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog

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  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67039-1-PBS bindet in WB, IHC, Indirect ELISA Flightless I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Flightless I fusion protein Ag26865
• Vollständiger Name: flightless I homolog (Drosophila)
• Berechnetes Molekulargewicht: 1269 aa, 145 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC025300
• Gene symbol: Flightless I
• Gene ID (NCBI): 2314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).