Flotillin 1 Rekombinanter Antikörper

Flotillin 1 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240582G6

Kat-Nr. : 83598-4-PBS

Synonyme

FLOT1, 240582G6, Flotillin-1

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  WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

  IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83598-4-RR (same clone as 83598-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83598-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 30.5 nM.

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Geprüfte Anwendungen

Produktinformation

83598-4-PBS bindet in WB, IHC, IF/ICC, IP, ELISA Flotillin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Flotillin 1 fusion protein Ag7902
• Vollständiger Name: flotillin 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001146
• Gene symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.