Foxp3 Monoklonaler Antikörper

Foxp3 Monoklonal Antikörper für IHC, FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

IHC, IF, FC (Intra)

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

3G3

Kat-Nr. : 65089-1-Ig

Synonyme

Foxp3, JM2, scurfin, sf, 3G3, forkhead box P3

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of C57BL/c mouse splenocytes using 65089-1-Ig

  1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

65089-1-Ig bindet in IHC, IF, FC (Intra) Foxp3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: forkhead box P3
• GenBank-Zugangsnummer: BC132333
• Gene symbol: Foxp3
• Gene ID (NCBI): 20371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publikationen

Species	Application	Title
mouse	IHC	Thorac Cancer; Adiponectin-expressing Treg cells in experimental thymic tumor model; Authors - Yuki Hanamatsu
mouse	IF	Adv Sci (Weinh); Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment; Authors - Yipeng Zhao
mouse	IF	Thorac Cancer; Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer; Authors - Wakana Chikaishi
		Cell Rep; FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation; Authors - Tongguan Tian
mouse	IF	Cell Commun Signal; Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma; Authors - Yuqi Yang
mouse	IHC	J Ethnopharmacol; The extract from Quzhou Aurantii Fructus attenuates cough variant asthma through inhibiting the TRPV1/Ca2+/NFAT/TSLP pathway and ferroptosis via TRPV1 mediation in ovalbumin-induced mice; Authors - Meizi Tian