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Foxp3 Monoklonaler Antikörper

Foxp3 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

APC Fluorescent Dye

CloneNo.

3G3

Kat-Nr. : APC-65089

Synonyme

forkhead box P3, Foxp3, JM2, scurfin, sf

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of BALB/c mouse splenocytes using APC Anti-Mouse Foxp3 (3G3) (APC-65089)

    FC experiment of BALB/c mouse splenocytes using APC-65089

    1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65089 bindet in FC (Intra) Foxp3 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name forkhead box P3
GenBank-ZugangsnummerBC132333
Gene symbol Foxp3
Gene ID (NCBI) 20371
Konjugation APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publikationen

SpeciesApplicationTitle
mouseFC (Intra)

Drug Deliv

Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.

Authors - Dingyi Wang
View Article
mouseFC (Intra)

Phytomedicine

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Authors - Li Wu
View Article
mouseFC (Intra)

ACS Nano

Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity

Authors - Yunkai Shang
View Article