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  • Featured Product
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Frizzled 5 Polyklonaler Antikörper

Frizzled 5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21519-1-PBS

Synonyme

FZD5, FzE5, Fz-5, Fz 5, Frizzled-5

Filter:
  • Western Blot (WB) analysis of various lysates using Frizzled 5 Polyclonal antibody (21519-1-AP)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Frizzled 5 Polyclonal antibody (21519-1-AP)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human stomach cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21519-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Frizzled 5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Frizzled 5 fusion protein Ag16082
Vollständiger Name frizzled homolog 5 (Drosophila)
Berechnetes Molekulargewicht 585 aa, 65 kDa
Beobachtetes Molekulargewicht 65-70 kDa
GenBank-ZugangsnummerBC114346
Gene symbol FZD5
Gene ID (NCBI) 7855
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.