G-CSF Rekombinanter Antikörper

G-CSF Rekombinant Antikörper für WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250389H1

Kat-Nr. : 86376-1-PBS

Synonyme

CSF3, C17orf33, CSF 3, FilgrastimLenograstim, GCSF

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  WB analysis using 86376-1-RR (same clone as 86376-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86376-1-RR (G-CSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86376-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02556-1

  Sandwich ELISA standard curve of MP02556-1, Human G-CSF Recombinant Matched Antibody Pair - PBS only. 86376-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1207. 86376-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

• 

  Sample test of MP02556-1

  Serum of eight individual healthy human donors was measured. The G-CSF concentration of detected samples was determined to be 40.3 pg/mL with a range of 11.8-197.6 pg/mL.

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  Sample test of MP02556-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean G-CSF concentration was determined to be 33.9 pg/mL in unstimulated PBMC supernatant, 858.2 pg/mL in PHA stimulated PBMC supernatant.

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  FC experiment of K-562 using 86376-1-RR (same clone as 86376-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug G-CSF Recombinant antibody (86376-1-RR, Clone:250389H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 86376-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86376-1-PBS bindet in WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test G-CSF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G-CSF fusion protein HZ-1207
• Vollständiger Name: colony stimulating factor 3 (granulocyte)
• Beobachtetes Molekulargewicht: 20 kDa
• Gene symbol: G-CSF
• Gene ID (NCBI): 1440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.