G-CSFR Polyklonaler Antikörper

G-CSFR Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 18310-1-AP

Synonyme

CD114, CSF3R, G CSF R, G CSF receptor, GCSFR, G-CSFR, G-CSF-R

---

Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U-937 using 18310-1-AP

  1X10^6 U-937 cells were stained with 0.2ug G-CSFR antibody (18310-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

18310-1-AP bindet in WB, FC,ELISA G-CSFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: colony stimulating factor 3 receptor (granulocyte)
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC053585
• Gene symbol: CSF3R
• Gene ID (NCBI): 1441
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; Ovarian cancer modulates the immunosuppressive function of CD11b+Gr1+ myeloid cells via glutamine metabolism.; Authors - Mary P Udumula
human	WB	J Cell Physiol; The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.; Authors - Yang Cheng