G-CSFR Rekombinanter Antikörper

G-CSFR Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242246A5

Kat-Nr. : 84827-2-RR

Synonyme

242246A5, CD114, CSF3R, G CSF R, G CSF receptor

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  WB analysis of THP-1 using 84827-2-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84827-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84827-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84827-2-RR (G-CSFR antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84827-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84827-2-RR (G-CSFR antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84827-2-RR bindet in WB, IHC, ELISA G-CSFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G-CSFR fusion protein Eg2821
• Vollständiger Name: colony stimulating factor 3 receptor (granulocyte)
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: NM_000760.3
• Gene symbol: G-CSFR
• Gene ID (NCBI): 1441
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.