G3BP1 Monoklonaler Antikörper

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G3BP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E4A2

Kat-Nr. : 66486-1-PBS

Synonyme

1E4A2, ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1

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  WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66486-1-Ig (same clone as 66486-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66486-1-Ig (same clone as 66486-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66486-1-Ig (same clone as 66486-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66486-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA G3BP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: G3BP1 fusion protein Ag3728
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 1
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC006997
• Gene symbol: G3BP1
• Gene ID (NCBI): 10146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.