G3BP1 Rekombinanter Antikörper

G3BP1 Rekombinant Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

7F14

Kat-Nr. : 80777-1-RR

Synonyme

7F14, ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1

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  IHC staining of mouse brain using 80777-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 80777-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 80777-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80777-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:1400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 80777-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80777-1-RR against Human G3BP1 were performed. The affinity constant is 7.07 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: sodium arsenite treated HeLa cells

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80777-1-RR bindet in IHC, IF/ICC, IF-P, ELISA G3BP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G3BP1 fusion protein Ag3728
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 1
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• GenBank-Zugangsnummer: BC006997
• Gene symbol: G3BP1
• Gene ID (NCBI): 10146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.