G3BP1 Monoklonaler Antikörper

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• KD/KO Validated

G3BP1 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IHC, IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

CloneNo.

1E4A2

Kat-Nr. : CL594-66486

Synonyme

G3BP, G3BP 1, G3BP1, HDH VIII

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  IF Staining of HeLa using CL594-66486

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®594 G3BP1 antibody (CL594-66486, Clone: 1E4A2 ) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of HeLa using CL594-66486

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66486 (G3BP1 antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: sodium arsenite treated HeLa cells, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

CL594-66486 bindet in IHC, IF/ICC G3BP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: G3BP1 fusion protein Ag3728
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 1
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC006997
• Gene symbol: G3BP1
• Gene ID (NCBI): 10146
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Publikationen

Species	Application	Title
mouse	IHC	J Mol Cell Cardiol; RBM20S639G mutation is a high genetic risk factor for premature death through RNA-protein condensates.; Authors - Chunyan Wang
	IHC	Nucleic Acids Res; A toolkit for the identification of NEAT1_2/paraspeckle modulators; Authors - Haiyan An