• Featured Product
  • KD/KO Validated

G3BP2 Polyklonaler Antikörper

G3BP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(27)

Kat-Nr. : 16276-1-AP

Synonyme

G3BP 2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2, G3BP-2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, C2C12-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Neuro-2a-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, Mauslebergewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCsodium arsenite treated HeLa cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16276-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA G3BP2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen G3BP2 fusion protein Ag9355
Vollständiger Name GTPase activating protein (SH3 domain) binding protein 2
Berechnetes Molekulargewicht482aa,54 kDa; 449aa,51 kDa
Beobachtetes Molekulargewicht65-70 kDa
GenBank-ZugangsnummerBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for G3BP2 antibody 16276-1-APProtokoll herunterladen
IHC protocol for G3BP2 antibody 16276-1-APProtokoll herunterladenl
IF protocol for G3BP2 antibody 16276-1-APProtokoll herunterladen
IP protocol for G3BP2 antibody 16276-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,CoIP

Cell

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

Authors - Qinqin Cui
human

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
humanWB,IP

Oncogene

HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer

Authors - Bei Liu
  • KD Validated
humanWB,IF,IP

Oncogene

RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.

Authors - Yaqi Chen
  • KD Validated
humanWB,IHC

Mol Cancer

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.

Authors - Hongwei Liu
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Karine (Verified Customer) (09-22-2022)

Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mM Na2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The protein concentration was measured with the DC Protein Assay (BIO-RAD) and 30μg of total proteins were loaded onto 12% SDS- polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots were incubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: H69 cells
G3BP2 Antibody Western Blot validation (1/1000 dilution) in H69 cells (Cat no:16276-1-AP)