G3BP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

G3BP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 16276-1-AP

Synonyme

G3BP 2, G3BP2, KIAA0660

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Galerie der Validierungsdaten

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  WB analysis using 16276-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16276-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T47D cells using 16276-1-AP

  WB result of G3BP2 antibody (16276-1-AP, 1:500) with T47D breast cancer cells.

• 

  IP experiment of HeLa using 16276-1-AP

  IP result of anti-G3BP2 (IP:16276-1-AP, 4ug; Detection:16276-1-AP 1:1000) with HeLa cells lysate 2200 ug.

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  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Neuro-2a-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Mauslebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: sodium arsenite treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

16276-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA G3BP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: G3BP2 fusion protein Ag9355
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 2
• Berechnetes Molekulargewicht: 482aa,54 kDa; 449aa,51 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC011731
• Gene symbol: G3BP2
• Gene ID (NCBI): 9908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Publikationen

Species	Application	Title
human		Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
human	WB,IP	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated
human	WB,IF,IP	Oncogene; RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.; Authors - Yaqi Chen ; KD Validated
human	WB,IHC	Mol Cancer; Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.; Authors - Hongwei Liu ; KD Validated
human	WB	Cancer Commun (Lond); BAALC-AS1/G3BP2/c-Myc feedback loop promotes cell proliferation in esophageal squamous cell carcinoma.; Authors - Hongyue Zhang
human	IP	J Virol; SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.; Authors - Hainan Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Karine (Verified Customer) (09-22-2022)

Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mMNa2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The proteinconcentration was measured with the DC Protein Assay (BIO-RAD) and 30μgof total proteins were loaded onto 12% SDS- polyacrylamide gels forelectrophoresis and transferred onto polyvinylidene difluoride membranes(Millipore). After 1h of blocking with 5% bovine serum albumin preparedin Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots wereincubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhancedchemiluminescence detection kit (Millipore) and and the Syngene Pxi4imaging system (Ozyme).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: H69 cells

G3BP2 Antibody Western Blot validation (1/1000 dilution) in H69 cells (Cat no:16276-1-AP)