G3BP2 Rekombinanter Antikörper

G3BP2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230275D6

Kat-Nr. : 82080-4-PBS

Synonyme

G3BP 2, 230275D6, G3BP-2, GAP SH3 domain binding protein 2, GAP SH3 domain-binding protein 2

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  WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00090-3

  The mean G3BP2 concentration was determined to be 8.91 ng/mL in A549 cell extract based on a 2.20 mg/mL extract load, 11.75 ng/mL in HeLa cell extract based on a 3.20 mg/mL extract load, 9.66 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 29.51 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00090-3

  Sandwich ELISA standard curve of MP00090-3, Human G3BP2 Recombinant Matched Antibody Pair - PBS only. 82080-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9355. 82080-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82080-4-RR (same clone as 82080-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-4-RR, Clone:230275D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00090-3

  Cytometric bead array standard curve of MP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 82080-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 82080-4-PBS against Human G3BP2 was performed. The affinity constant is 3.48 nM.

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  Affinity and Kinetic Characterization of 82080-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

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Geprüfte Anwendungen

Produktinformation

82080-4-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA G3BP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G3BP2 fusion protein Ag9355
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 2
• Berechnetes Molekulargewicht: 482aa,54 kDa; 449aa,51 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC011731
• Gene symbol: G3BP2
• Gene ID (NCBI): 9908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.