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G6PC Polyklonaler Antikörper

G6PC Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29084-1-PBS

Synonyme

G6PC1, EC:3.1.3.9, G6Pase, G-6-Pase, G6Pase-alpha

Filter:
  • Western Blot (WB) analysis of various lysates using G6PC Polyclonal antibody (29084-1-AP)

    WB analysis using 29084-1-AP (same clone as 29084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29084-1-AP (G6PC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using G6PC Polyclonal antibody (29084-1-AP)

    IF Staining of mouse liver using 29084-1-AP (same clone as 29084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (29084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29084-1-PBS bindet in WB, IF-P, Indirect ELISA G6PC und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen G6PC fusion protein Ag30614
Vollständiger Name glucose-6-phosphatase, catalytic subunit
Berechnetes Molekulargewicht 357 aa, 40 kDa
Beobachtetes Molekulargewicht 40 kDa
GenBank-ZugangsnummerBC130478
Gene symbol G6PC
Gene ID (NCBI) 2538
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).