G6PC Monoklonaler Antikörper

G6PC Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E11A5

Kat-Nr. : 66860-1-PBS

Synonyme

G6PC1, 1E11A5, EC:3.1.3.9, G6Pase, G-6-Pase

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  WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66860-1-Ig (same clone as 66860-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (66860-1-Ig, Clone: 1E11A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50826-2

  Cytometric bead array standard curve of MP50826-2, G6PC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66860-4-PBS. Detection antibody: 66860-1-PBS. Standard:Ag17839. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66860-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA G6PC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: G6PC fusion protein Ag17839
• Vollständiger Name: glucose-6-phosphatase, catalytic subunit
• Berechnetes Molekulargewicht: 357 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-42 kDa
• GenBank-Zugangsnummer: BC130478
• Gene symbol: G6PC
• Gene ID (NCBI): 2538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).