G6PD Rekombinanter Antikörper

G6PD Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250831F2

Kat-Nr. : 86240-2-RR

Synonyme

EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

---

• 

  WB analysis using 86240-2-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86240-2-RR (G6PD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86240-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86240-2-RR against Human G6PD were performed. The affinity constant is 15.5 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HT-29-Zellen, MCF-7-Zellen, NIH-3T3-Zellen, Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86240-2-RR bindet in WB, ELISA G6PD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: G6PD fusion protein Ag21862
• Vollständiger Name: glucose-6-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000337
• Gene symbol: G6PD
• Gene ID (NCBI): 2539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.