GAA Polyklonaler Antikörper

GAA Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 14367-1-AP

Synonyme

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase

---

Galerie der Validierungsdaten

• 

  WB analysis using 14367-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14367-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14367-1-AP

  IP Result of anti-GAA (IP:14367-1-AP, 4ug; Detection:14367-1-AP 1:600) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 4 publications below

Produktinformation

14367-1-AP bindet in WB, IP, IHC, IF, ELISA GAA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAA fusion protein Ag5690
• Vollständiger Name: glucosidase, alpha; acid
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 70-76 kDa
• GenBank-Zugangsnummer: BC040431
• Gene symbol: GAA
• Gene ID (NCBI): 2548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis; Authors - Lindsey R Conroy
human	IF	J Med Chem; 5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease.; Authors - Atsushi Kato
human	IF	Org Biomol Chem; Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation; Authors - Atsushi Kato
zebrafish	WB	DNA Cell Biol; Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease.; Authors - Jing Wu
	IF	J Med Chem; Design and Pharmacological Chaperone Effects of N-(4'-Phenylbutyl)-DAB Derivatives Targeting the Lipophilic Pocket of Lysosomal Acid α-Glucosidase; Authors - Atsushi Kato

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

CHRISTINE (Verified Customer) (09-27-2019)

will continue to order here in the future.

• Applications: Western Blot, Flow Cytometry, Cell culture,
• Cell Tissue Type: IgG