GAA Polyklonaler Antikörper

GAA Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29993-1-PBS

Synonyme

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  WB analysis using 29993-1-AP (same clone as 29993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29993-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29993-1-PBS bindet in WB, IHC, Indirect ELISA GAA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAA fusion protein Ag28970
• Vollständiger Name: glucosidase, alpha; acid
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC040431
• Gene symbol: GAA
• Gene ID (NCBI): 2548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.