GAB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GAB2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22549-1-PBS

Synonyme

---

• 

  WB analysis using 22549-1-AP (same clone as 22549-1-PBS)

  K-562 cells lysates were subjected to SDS PAGE followed by western blot with 22549-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 22549-1-AP (same clone as 22549-1-PBS)

  WB result of GAB2 antibody (22549-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 22549-1-AP (same clone as 22549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 22549-1-AP (same clone as 22549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 22549-1-AP (same clone as 22549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GAB2 antibody (22549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

22549-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GAB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAB2 fusion protein Ag18192
• Vollständiger Name: GRB2-associated binding protein 2
• Berechnetes Molekulargewicht: 676 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74-90 kDa
• GenBank-Zugangsnummer: BC131711
• Gene symbol: GAB2
• Gene ID (NCBI): 9846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.