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GABARAP Polyklonaler Antikörper
GABARAP Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18723-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, A549-Zellen, HuH-7-Zellen, humanes Herzgewebe, humanes Plazenta-Gewebe, Maushirngewebe, Mauslebergewebe, Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, RAW 264.7-Zellen, SH-SY5Y-Zellen | 
| Erfolgreiche IP | HeLa-Zellen | 
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, Ausgehungerte HepG2-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below | 
| WB | See 22 publications below | 
| IF | See 6 publications below | 
| CoIP | See 5 publications below | 
Produktinformation
18723-1-AP bindet in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA GABARAP und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus | 
| In Publikationen genannte Reaktivität | human, Affe, Maus | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | GABA(A) receptor-associated protein | 
| Berechnetes Molekulargewicht | 14 kDa | 
| Beobachtetes Molekulargewicht | 14-18 kDa | 
| GenBank-Zugangsnummer | BC106748 | 
| Gene symbol | GABARAP | 
| Gene ID (NCBI) | 11337 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for GABARAP antibody 18723-1-AP | Protokoll herunterladen | 
| IF protocol for GABARAP antibody 18723-1-AP | Protokoll herunterladen | 
| IP protocol for GABARAP antibody 18723-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||
| Autophagy ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis
 | ||
| Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1
 | ||
| Autophagy ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Hala (Verified Customer) (05-09-2023) | works very good in milk 5% 
  | 
| FH Hala (Verified Customer) (04-28-2023) | works very good with milk blocking 
 | 
| FH Anita (Verified Customer) (12-22-2020) | HeLa cell protein extract (5 micrograms) 
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