GABARAP Polyklonaler Antikörper

GABARAP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 22439-1-AP

Synonyme

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B

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  WB analysis using 22439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22439-1-AP

  Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22439-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22439-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 22439-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22439-1-AP (GABARAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, mit Chloroquin behandelte HEK-293-Zellen, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22439-1-AP bindet in WB, IHC, ELISA GABARAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABARAP fusion protein Ag18209
• Vollständiger Name: GABA(A) receptor-associated protein
• Berechnetes Molekulargewicht: 117 aa, 14 kDa
• Beobachtetes Molekulargewicht: 19-21 kDa
• GenBank-Zugangsnummer: BC106749
• Gene symbol: GABARAP
• Gene ID (NCBI): 11337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other