GABARAPL1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

GABARAPL1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E10F6

Kat-Nr. : 66458-1-PBS

Synonyme

1E10F6, APG8 LIKE, APG8L, ATG8, ATG8L

---

• 

  WB analysis of mouse brain using 66458-1-Ig (same clone as 66458-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66458-1-Ig (same clone as 66458-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66458-1-Ig (same clone as 66458-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66458-1-Ig (same clone as 66458-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66458-1-PBS bindet in WB, IF/ICC, ELISA GABARAPL1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GABARAPL1 fusion protein Ag1473
• Vollständiger Name: GABA(A) receptor-associated protein like 1
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC009309
• Gene symbol: GABARAPL1
• Gene ID (NCBI): 23710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.