GABARAPL2-Specific Polyclonal antibody

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• GABARAPL2-Specific Polyclonal antibody (18724-1-AP)

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• KD/KO Validated

GABARAPL2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 18724-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

GABARAPL2, ATG8, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2

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  WB analysis of COLO 320 using 18724-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 18724-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 18724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 18724-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 18724-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 18724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL2-Specific antibody (18724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 18724-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL2-Specific Polyclonal antibody (18724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Chloroquine treated HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18724-1-AP targets GABARAPL2-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GABA(A) receptor-associated protein-like 2
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005985
• Gene Symbol: GABARAPL2
• Gene ID (NCBI): 11345
• RRID: AB_2232116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60520
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Publications

What published studies show

The GABARAPL2-Specific Polyclonal antibody (Cat# 18724-1-AP) has 18 citations across 12 journals, including Nature Immunology, Autophagy, Developmental Cell, Cell Death & Disease, Experimental & Molecular Medicine, Frontiers in Immunology, Journal of Immunology, FASEB Journal, Scientific Reports, Neurobiology of Disease, Molecular Cell Biology, and Computers in Biology and Medicine. Research fields span autophagy, immune defense, neurodegeneration, cancer, viral infection, and mitochondrial/lysosomal biology.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai ; KO Validated
human	WB	Autophagy; A fetus with severe developmental defects caused by dominant-negative and hypomorphic ATG7 alleles.; Authors - Marion Carpentier
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
rat	WB	Autophagy; CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.; Authors - Hyun-Kyu An
	WB	Autophagy; TMEM184A-mediated autophagy in MHC-I degradation promotes tumor immune evasion; Authors - Wenyi Li
rat	WB	Exp Mol Med; GSK3B induces autophagy by phosphorylating ULK1.; Authors - Hye Young Ryu