GABARAPL2-Specific Polyklonaler Antikörper

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• KD/KO Validated

GABARAPL2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18724-1-PBS

Synonyme

GABARAPL2, ATG8, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2

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  WB analysis of COLO 320 using 18724-1-AP (same clone as 18724-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 18724-1-AP (same clone as 18724-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 18724-1-AP (same clone as 18724-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 18724-1-AP (same clone as 18724-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 18724-1-AP (same clone as 18724-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 18724-1-AP (same clone as 18724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL2-Specific antibody (18724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 18724-1-AP (same clone as 18724-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL2-Specific Polyclonal antibody (18724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18724-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18724-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA GABARAPL2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GABA(A) receptor-associated protein-like 2
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005985
• Gene symbol: GABARAPL2
• Gene ID (NCBI): 11345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.