GABBR2 Polyklonaler Antikörper

GABBR2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (1)

Kat-Nr. : 27567-1-AP

Synonyme

G protein coupled receptor 51, GABA B R2, GABA B receptor 2, GABA BR2, GABABR2, GABBR2, Gb2, GPR51, GPRC3B, HG20, HRIHFB2099

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• 

  WB analysis using 27567-1-AP

  mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 27567-1-AP (GABBR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 27567-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27567-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

27567-1-AP bindet in WB, IHC, IF, ELISA GABBR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABBR2 fusion protein Ag25886
• Vollständiger Name: gamma-aminobutyric acid (GABA) B receptor, 2
• Berechnetes Molekulargewicht: 941 aa, 106 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC035071
• Gene symbol: GABBR2
• Gene ID (NCBI): 9568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang
human	WB	World J Gastroenterol; γ-aminobutyric acid B2 receptor: A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus; Authors - Charupong Saengboonmee
human	WB,IHC	Biochem Biophys Res Commun; GAD1-mediated GABA elicits aggressive characteristics of human oral cancer cells; Authors - Zhichao Dou
human	IHC	Life Sci; GABA regulates the proliferation and apoptosis of head and neck squamous cell carcinoma cells by promoting the expression of CCND2 and BCL2L1; Authors - Kunliang Luo
rat	WB	Neurorehabil Neural Repair; Metabolomics-Based Effects of a Natural Product on Remyelination After Cerebral Ischemia Injury Via GABABR-pCREB-BDNF Pathway; Authors - Xiaodi Fan
mouse	WB	Behav Brain Res; Impact of Abl2/Arg Deficiency on Anxiety and Depressive Behaviors in mice; Authors - Xiaojuan Yao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

De (Verified Customer) (02-18-2020)

Got positive WB results in B6 mouse brain tissues by using this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: tissue