GABPB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GABPB1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12597-1-AP

Synonyme

BABPB2, E4TF1, E4TF1 47, E4TF1 53, E4TF1B, GABP subunit beta 1, GABP subunit beta 2, GABPB, GABPB 1, GABPB 2, GABPB1, GABPB2, NRF2B1, NRF2B2, Nuclear respiratory factor 2, Transcription factor E4TF1 47, Transcription factor E4TF1 53

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 12597-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12597-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12597-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12597-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12597-1-AP

  IP Result of anti-GABPB1 (IP:12597-1-AP, 4ug; Detection:12597-1-AP 1:1000) with HeLa cells lysate 520ug.

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 12597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPB1 antibody (12597-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12597-1-AP bindet in WB, IP, IHC, IF, ELISA GABPB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABPB1 fusion protein Ag3208
• Vollständiger Name: GA binding protein transcription factor, beta subunit 1
• Berechnetes Molekulargewicht: 347 aa, 38 kDa
• Beobachtetes Molekulargewicht: 36-45 kDa, 47-53 kDa
• GenBank-Zugangsnummer: BC016910
• Gene symbol: GABPB1
• Gene ID (NCBI): 2553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.; Authors - Andrew Mancini ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.; Authors - Alexandra M Amen
human, mouse	WB,IHC,IF	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H ; KD Validated
human	WB	Sci Rep; LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.; Authors - Wenchuan Qi ; KD Validated
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang
mouse	WB	Brain Res Bull; ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway.; Authors - Hengjiang Guo