GABPB2 Polyklonaler Antikörper

GABPB2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20870-1-AP

Synonyme

GABPB-2, GABPB 2, GABP subunit beta-2, GABP subunit beta 2, GA-binding protein subunit beta-2

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  WB analysis of Jurkat using 20870-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20870-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20870-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A375-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IP: See 1 publications below

Produktinformation

20870-1-AP bindet in WB, IP, ELISA GABPB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABPB2 fusion protein Ag14933
• Vollständiger Name: GA binding protein transcription factor, beta subunit 2
• Berechnetes Molekulargewicht: 448 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC027033
• Gene symbol: GABPB2
• Gene ID (NCBI): 126626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABPB2 is a subunit of the GA-binding protein (GABP) transcription factor, which is a heterodimeric complex. The GABP complex plays a role in the regulation of gene transcription by binding to specific DNA sequences. GABPB2 specifically contributes to the transactivation of target genes (PMID: 18628204). The protein is primarily located in the nucleus. It is ubiquitously expressed in various tissues, including testis, ovary, and others. Studies have shown that GABPB2 is dispensable for normal lymphocyte development but moderately affects B cell responses (PMID: 18628204). Mice deficient in GABPB2 exhibit increased B cell proliferation and antibody production in response to certain stimuli. The molecular weight of GABPB2 is 48 kDa.

Publikationen

Species	Application	Title
human	IP	Mol Cell; Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas; Authors - Zhumei Shi ; KD Validated