GABRA1 Polyklonaler Antikörper

GABRA1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(41)

Rezensionen (1)

Kat-Nr. : 12410-1-AP

Synonyme

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1

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  WB analysis using 12410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12410-1-AP (GABRA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12410-1-AP

  IP result of anti-GABRA1 (IP:12410-1-AP, 3ug; Detection:12410-1-AP 1:400) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 32 publications below
• IHC: See 2 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

12410-1-AP bindet in WB, IHC, IF, IP, ELISA GABRA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABRA1 fusion protein Ag3117
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, alpha 1
• Berechnetes Molekulargewicht: 456 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC030696
• Gene symbol: GABRA1
• Gene ID (NCBI): 2554
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human		Neurol Neuroimmunol Neuroinflamm; MRI Characteristics of Autoimmune Encephalitis With Autoantibodies to GABAA Receptor: A Case Series.; Authors - Bo Deng
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
mouse	WB	Int J Mol Sci; The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy.; Authors - Mariusz Popek

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (11-05-2024)

This gabra1 antibody works perfectly, the best I have tried so far. It shows perfectly the puncta on the axons of gabaergic neurons on the upper layers of the cortex.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

GABRA1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12410-1-AP)