GABRA2 Rekombinanter Antikörper

GABRA2 Rekombinant Antikörper für FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230158H5

Kat-Nr. : 83057-4-PBS

Synonyme

230158H5, GABA(A) receptor subunit alpha-2, GABAAR subunit alpha-2, Gamma-aminobutyric acid receptor subunit alpha-2

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  FC experiment of HeLa using 83057-4-RR (same clone as 83057-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-4-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83057-4-RR (same clone as 83057-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00067-3

  Cytometric bead array standard curve of MP00067-3, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-2-PBS. Detection antibody: 83057-4-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

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  Affinity and Kinetic Characterization of 83057-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83057-4-PBS against Human GABRA2 was performed. The affinity constant is 0.421 nM.

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  Affinity and Kinetic Characterization of 83057-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83057-4-RR against Human GABRA2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83057-4-PBS bindet in FC (Intra), Cytometric bead array, Indirect ELISA GABRA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GABRA2 fusion protein Ag29301
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-51 kDa
• GenBank-Zugangsnummer: NM_000807
• Gene symbol: GABRA2
• Gene ID (NCBI): 2555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.