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GABRG2 Polyklonaler Antikörper

GABRG2 Polyklonal Antikörper für WB, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14104-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using GABRG2 Polyclonal antibody (14104-1-AP)

    WB analysis using 14104-1-AP (same clone as 14104-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GABRG2 Polyclonal antibody (14104-1-AP)

    IP experiment of mouse brain using 14104-1-AP (same clone as 14104-1-PBS)

    IP result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using GABRG2 Polyclonal antibody (14104-1-AP)

    IF Staining of mouse cerebellum using 14104-1-AP (same clone as 14104-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14104-1-PBS bindet in WB, IF-P, IP, Indirect ELISA GABRG2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GABRG2 fusion protein Ag5237
Vollständiger Name gamma-aminobutyric acid (GABA) A receptor, gamma 2
Berechnetes Molekulargewicht 467 aa, 54 kDa
Beobachtetes Molekulargewicht 54-60 kDa
GenBank-ZugangsnummerBC059389
Gene symbol GABRG2
Gene ID (NCBI) 2566
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.