GAC-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GAC-specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 19958-1-AP

Synonyme

GLS, KGA, GLS1, GAM, GAC

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  WB analysis of A549 using 19958-1-AP

  WB result of GAC-specific antibody (19958-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAC-specific transfected A549 cells.

• 

  WB analysis using 19958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of mouse kidney using 19958-1-AP

  IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 19958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GAC-specific antibody (19958-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

19958-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA GAC-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: AF158555
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection.; Authors - Maria L Balmer
mouse	IF	Cell Stem Cell; Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.; Authors - Pia Sommerkamp
human	WB,IP	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB	Blood; Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.; Authors - Nathalie Jacque
	WB	Elife; Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor; Authors - Carolyn M Jablonowski
human	WB,IHC	Cell Death Discov; O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma; Authors - Sijia Fang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK2 cells sustained on glucose or galactose

GAC-specific Antibody Western Blot, validation (1:1000 dilution) in HK2 cells sustained on glucose or galactose (Cat no:19958-1-AP)