GAD1 Polyklonaler Antikörper

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GAD1 Polyklonal Antikörper für WB, IHC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10408-1-PBS

Synonyme

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  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10408-1-PBS bindet in WB, IHC, IF-Fro, IP, ELISA GAD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAD1 fusion protein Ag0666
• Vollständiger Name: glutamate decarboxylase 1 (brain, 67kDa)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC002815
• Gene symbol: GAD1
• Gene ID (NCBI): 2571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

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