GAD1 Monoklonaler Antikörper

GAD1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

2D5B7

Rezensionen (1)

Kat-Nr. : 67648-1-Ig

Synonyme

2D5B7, EC:4.1.1.15, FLJ45882, GAD, GAD 1

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  WB analysis using 67648-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of pig brain using 67648-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67648-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67648-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 67648-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Cerebellum-Gewebe, Kaninchenhirngewebe, Kaninchen-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Ratten-Cerebellum-Gewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

67648-1-Ig bindet in WB, IHC, IF-P, ELISA GAD1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAD1 fusion protein Ag21336
• Vollständiger Name: glutamate decarboxylase 1 (brain, 67kDa)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 57 kDa
• GenBank-Zugangsnummer: BC002815
• Gene symbol: GAD1
• Gene ID (NCBI): 2571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.

Publikationen

Species	Application	Title
mouse	WB	Biol Trace Elem Res; Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice; Authors - Mengjie Qi
rat	IHC	Acta Histochem; Proposal for a simple and easy-to-implement protocol for three-dimensional tissue imaging that is compatible with observation using a confocal microscope; Authors - Takuto Matano
human	WB	Dev Cell; The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis; Authors - Li Li
mouse	IF	Acta Pharmacol Sin; Differential regulation of pruritic sensation and emotion by cannabinoid type 1 receptors on mPFC glutamatergic and GABAergic neurons; Authors - Ou-Yang Zhanmu
mouse	IF	J Psychiatr Res; NLRP3 in the GABAergic neuron induces cognitive impairments in a mouse model of hemorrhage shock and resuscitation; Authors - Ji-Zhen Liu
mouse,human	WB,IF	Cells; CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury; Authors - Yanbing Kao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (07-29-2024)

This is the first time a GAD antibody (in green) works properly in a human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

GAD1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:67648-1-Ig)