GAD1 Monoklonaler Antikörper

GAD1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5B7

Kat-Nr. : 67648-1-PBS

Synonyme

2D5B7, EC:4.1.1.15, FLJ45882, GAD, GAD 1

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  WB analysis of pig brain using 67648-1-Ig (same clone as 67648-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67648-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA GAD1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAD1 fusion protein Ag21336
• Vollständiger Name: glutamate decarboxylase 1 (brain, 67kDa)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 57 kDa
• GenBank-Zugangsnummer: BC002815
• Gene symbol: GAD1
• Gene ID (NCBI): 2571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.