GAD65 Polyklonaler Antikörper

GAD65 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21760-1-AP

Synonyme

GAD 65, GAD2, GAD65, Glutamate decarboxylase 2

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Galerie der Validierungsdaten

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  WB analysis using 21760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21760-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21760-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 21760-1-AP

  IP Result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21760-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAD65 antibody (21760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21760-1-AP (GAD65 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:100-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

21760-1-AP bindet in WB, IP, IHC, IF, ELISA GAD65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAD65 fusion protein Ag16251
• Vollständiger Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC126327
• Gene symbol: GAD2
• Gene ID (NCBI): 2572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
mouse	WB	J Neuroinflammation; FKBP51 mediates resilience to inflammation-induced anxiety through regulation of glutamic acid decarboxylase 65 expression in mouse hippocampus.; Authors - Yu-Ling Gan
human	WB	Neuropharmacology; Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.; Authors - Yue Li
mouse	WB	Neurosci Lett; Repeated maternal separation prolongs the critical period of ocular dominance plasticity in mouse visual cortex.; Authors - Sitong Li