GAD65 Polyklonaler Antikörper

GAD65 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21760-1-PBS

Synonyme

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  WB analysis using 21760-1-AP (same clone as 21760-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 21760-1-AP (same clone as 21760-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat brain using 21760-1-AP (same clone as 21760-1-PBS)

  IP result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GAD65 antibody (21760-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21760-1-AP (same clone as 21760-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAD65 antibody (21760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21760-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA GAD65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAD65 fusion protein Ag16251
• Vollständiger Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC126327
• Gene symbol: GAD65
• Gene ID (NCBI): 2572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.