GADD45A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GADD45A Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Rezensionen (1)

Kat-Nr. : 13747-1-AP

Synonyme

DDIT 1, DDIT1, GADD45, GADD45A

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• 

  WB analysis using 13747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13747-1-AP bindet in WB, IHC, CoIP, ELISA GADD45A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GADD45A fusion protein Ag4687
• Vollständiger Name: growth arrest and DNA-damage-inducible, alpha
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa, 33 kDa
• GenBank-Zugangsnummer: BC011757
• Gene symbol: GADD45A
• Gene ID (NCBI): 1647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

Publikationen

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Phytother Res; Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma; Authors - Ronghui Fan
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han
human	WB	Cells; Unraveling the Pathogenetic Mechanisms Underlying the Association between Specific Mitochondrial DNA Haplogroups and Parkinson's Disease; Authors - Min-Yu Lan
human	WB	Sci Rep; Down-regulation of GADD45A enhances chemosensitivity in melanoma.; Authors - Jia Liu
human	WB,IHC	J Exp Clin Cancer Res; Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway.; Authors - Gangjun Yuan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-27-2025)

Antibody works well when diluted in 1x TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver tissue

GADD45A Antibody Western Blot validation (1:1000 dilution) in Mouse Liver tissue (Cat no:13747-1-AP)