GADD45A Rekombinanter Antikörper

GADD45A Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241597F5

Kat-Nr. : 84376-3-PBS

Synonyme

241597F5, DDIT 1, DDIT1, DDIT-1, DNA damage-inducible transcript 1 protein

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  IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84376-3-RR (same clone as 84376-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GADD45A antibody (84376-3-RR, Clone: 241597F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 84376-3-RR (same clone as 84376-3-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-3-RR, Clone:241597F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01247-3

  Cytometric bead array standard curve of MP01247-3, GADD45A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84376-2-PBS. Detection antibody: 84376-3-PBS. Standard: Ag4687. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84376-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84376-3-RR against Human GADD45A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84376-3-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA GADD45A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GADD45A fusion protein Ag4687
• Vollständiger Name: growth arrest and DNA-damage-inducible, alpha
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• GenBank-Zugangsnummer: BC011757
• Gene symbol: GADD45A
• Gene ID (NCBI): 1647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.