GADD45A Rekombinanter Antikörper

GADD45A Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241597H4

Kat-Nr. : 84376-4-RR

Synonyme

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

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  WB analysis using 84376-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of K562 using 84376-4-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 84376-4-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84376-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS, K562

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84376-4-RR bindet in WB, FC (Intra), ELISA GADD45A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GADD45A fusion protein Ag4687
• Vollständiger Name: growth arrest and DNA-damage-inducible, alpha
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 14-18 kDa, 28-36 kDa
• GenBank-Zugangsnummer: BC011757
• Gene symbol: GADD45A
• Gene ID (NCBI): 1647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).