GADD45GIP1 Polyklonaler Antikörper

GADD45GIP1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16260-1-AP

Synonyme

CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16260-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 16260-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16260-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16260-1-AP

  IP Result of anti-GADD45GIP1 (IP:16260-1-AP, 4ug; Detection:16260-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, NIH/3T3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16260-1-AP bindet in WB, IP, IHC, IF,ELISA GADD45GIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GADD45GIP1 fusion protein Ag9299
• Vollständiger Name: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC013027
• Gene symbol: GADD45GIP1
• Gene ID (NCBI): 90480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Science; T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.; Authors - Isobel Okoye
human	IHC	Oncotarget; CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.; Authors - Runzhou Zhuang
rat	WB	J Mol Med (Berl); Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.; Authors - Li Peng
mouse	WB	Burns Trauma; Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.; Authors - Lingfei Li