GADD45GIP1 Polyklonaler Antikörper

GADD45GIP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16260-1-AP

Synonyme

Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2

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  WB analysis using 16260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16260-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 16260-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16260-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16260-1-AP

  IP result of anti-GADD45GIP1 (IP:16260-1-AP, 4ug; Detection:16260-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Mausherzgewebe, NIH/3T3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16260-1-AP bindet in WB, IHC, IF, IP, ELISA GADD45GIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GADD45GIP1 fusion protein Ag9299
• Vollständiger Name: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC013027
• Gene symbol: GADD45GIP1
• Gene ID (NCBI): 90480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Science; T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.; Authors - Isobel Okoye
		Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
human	IHC	Oncotarget; CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.; Authors - Runzhou Zhuang
rat	WB	J Mol Med (Berl); Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.; Authors - Li Peng
mouse	WB	Burns Trauma; Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.; Authors - Lingfei Li
mouse	WB,IHC	Int J Mol Sci; Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1; Authors - Kazuhiro Hasegawa